Izzy Christiansen and Jordan Nobbs have withdrawn from England’s squad for Friday’s World Cup qualifier in Russia.
Manchester City forward Christiansen has a thigh injury, while Arsenal midfielder Nobbs has a foot problem.
Sunderland skipper Lucy Staniforth and Lauren Bruton of Reading, who are both uncapped, have been called up as replacements by boss Phil Neville.
The Lionesses were already without captain Steph Houghton, who pulled out on Monday to have knee surgery.
England women are top of their qualifying group for France 2019, two points clear of second-placed Wales with three games to play.
Staniforth, 25, said she was “buzzing” to be involved with England again, after previously being called up under former boss Mark Sampson.
The midfielder, who has overcome two serious cruciate knee ligament injuries, posted on social media “So proud, an honour to represent my country!”
Having started her career at Sunderland, Staniforth rejoined them two years ago after an injury-hit spell at Liverpool.
She is one of six players in the England squad – which also includes Houghton, Lucy Bronze, Jill Scott, Carly Telford and Beth Mead – to have played for the Black Cats, who finished seventh in Women’s Super League 1 but are set to be demoted to the third tier after a league restructure.
The club is appealing against the decision.