Defending champion Jelena Ostapenko and seven-time Grand Slam winner Venus Williams went out of the French Open in the first round on Sunday.
American ninth seed Williams, a finalist at Roland Garros in 2002, was beaten 6-4 7-5 by world number 91 Wang Qiang of China.
Latvian fifth seed Ostapenko was stunned by Ukrainian Kateryna Kozlova, who came through 7-5 6-3.
Meanwhile, fourth seed Elina Svitolina beat Ajla Tomljanovic 7-5 6-3.
Williams, 37, is set to compete in the doubles with sister Serena in Paris later in the tournament.
At 19 years old, the then unseeded Ostapenko pulled off a massive coup by beating current world number one Simona Halep to take the title last year.
But this time around, she was beaten in 95 minutes on Philippe Chatrier by the world number 66, who had never triumphed over a top-30 player.
Britain’s Johanna Konta was also a first-round casualty, going down in straight sets against world number 93 Yulia Putintseva, who won 6-4 6-3 in an hour and 24 minutes.
World number 10 Sloane Stephens, who won the US Open last year, breezed into the second round by beating Arantxa Rus of the Netherlands 6-2 6-0.