During the news conference, Mr. Okamura, Ms. Le Pen and Mr. Wilders accused the bloc of destroying the sovereignty of member states by adopting a mandatory quota for asylum seekers from the Middle East.
“Because we like Europe, we say that the E.U. is going to kill her,” said Ms. Le Pen, president of the National Front and a former French presidential candidate. “We are patriots; we defend our sovereignty,” she added.
The Muslim community in the Czech Republic is between 5,000 and 20,000, or less than 0.02 percent of the total population. So far, the country has taken in just 12 asylum seekers as part of the European Union quota. The bloc plans to take the Czech Republic, Hungary and Poland to court for failing to accommodate their fair share of refugees under a plan agreed to by the 28-country body.
Mr. Wilders said he hoped the Czech capital would keep its doors closed to migrants.
As in neighboring Poland and Hungary, anti-immigrant and anti-establishment rhetoric has become a mainstay in politics in the Czech Republic, helping the eccentric billionaire and Euro-skeptic Andrej Babis to become prime minister in the last elections.
While he is also staunchly against the European Union’s migrant quotas, he pledged during his campaign not to cooperate with the far-right groups. But since taking office this past week without a majority in Parliament to rule outright, he has shown a willingness to work with Mr. Okamura and his SPD party on a quid pro quo basis.
Several leftist groups that opposed the meeting in Prague organized protests on Friday and Saturday in the center of the capital and on the outskirts of city, where the conference took place. About 400 people gathered Saturday outside the news conference, chanting, whistling and booing far-right supporters as they entered the compound, guarded by security and police officers.
“In the Czech Republic, there is an increasing number of attacks against minorities due to the policies of the Czech SPD party,” said Jan Cemper, the protests’ organizer. “At the same time, we want to show that such congresses of xenophobes and populists are not welcomed here.”
Fedor Gal, a former dissident and a public figure in the Czech Republic and Slovakia, referring to extreme right politicians, said, “Those are people that do not care for a dialogue, and they do not deserve our tolerance.”
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Czech politicians condemned the conference. Jiri Pospisil, chairman of TOP 09, a center-right party, wrote in a Facebook post: “Extremists that threaten the interests of both the Czech Republic and Europe gather in Prague. They want to leave the EU and NATO and bring us close to Russia of Vladimir Putin. What a shame that the host is vice chairman of the Czech parliament.”
Security measures were tight for the far-right politicians, particularly Mr. Wilders, who has faced threats. No violence was reported, however, and the police did not intervene in the demonstrations.
Though no public agenda for the conference was made public, the group introduced the Belgian right-wing politician Gerolf Annemans as the Europe of Nations and Freedom’s new president and visited the Czech Parliament on a guided tour.
Also expected at the conference were Janice Atkinson, an independent member of the European Parliament who was expelled from Britain’s populist U.K.I.P. party two years ago amid a financing scandal; and Marcus Pretzell, a German member of the European Parliament formerly with the Alternative for Germany party, known as the AfD.